The first update to the MOZ1 assembly, MOZ2 involved the results of a concerted effort to correct some of the ambiguities in scaffold map locations and orientations by manual analysis of the archived BAC chromosome hybridization photographs and by the hybridization of a small number of new BAC clones selected to resolve questions of scaffold orientation. The new AGP file, and early draft of which was first displayed on the A. gambiae genome poster published in the 4 October 2002 issue of science, formed the basis of a new annotation and gene build displayed on 1 October 2003 (MOZ2). This assembly was also 278 Mb.


The A. gambiae S Pimperena colony was established from blood-fed adult females collected in the village of Pimperena, Mali in November 2005. Approximately 5 isofemale families molecularly identified as A. gambiae S form were used to establish the colony.


The Anopheles gambiae PEST strain was chosen for genome sequencing because it had both a fixed, standard chromosomal arrangement and a sex-linked pink eye mutation that could readily be used as an indicator of cross-colony contamination. The pink eye mutation originated in a colony called A. gambiae LPE established in 1951 at the London School of Hygiene and Tropical Medicine from mosquitoes collected in Lagos, Nigeria.


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