Annotation of the AgamP3 assembly was carried out by VectorBase. The set of gene models presented (genebuild AgamP3.6, released December 2010) combines manual annotation of chromosome arm 2L,2R & X, data provided by the research community, and gene prediction using the Ensembl system. Prediction utilised alignments of dipteran and other protein sets to the genome and generation of GeneWise models, alignment and gene prediction based on Anopheles ESTs, and selected ab initio predictions. 'Known' genes are those which could be named using entries from the community gene symbol database or from UniProt.